accutane 30 mg sales In vitro studies with long term oestrogen deprived cells LTED, a line of MCF7 breast cancer cells developed under oestrogen deprived conditions, have shown that these breast cancer cells adapted to endocrine deprivation by becoming hypersensitive to oestradiol doses as low as 10 12 M Martin et al, 2003, Santen et al, 2005